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Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
Methyljasmonate accelerates catabolism of monoterpenoid indole alkaloids in Catharanthus roseus during leaf
Magdi El-Sayed1, Rob Verpoorte
1Department of Pharmacognosy, Section of Metabolomics, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands. m.el-sayed@chem.leidenuniv.nl
Abstract:
Variations in alkaloid pattern during drying of leaves (leaf processing) showed that treatment with methyljasmonate can induce formation of bisindole alkaloids as a result of catabolism of the monomeric alkaloids catharanthine and vindoline. A two-fold increase in 3',4'-anhydrovinblastine was shown in treated leaves especially from day 8 until day 21. Serpentine also increased in the same period under the treatment as a catabolic product of ajmalicine. Basic peroxidases that are responsible for the formation of anhydrovinblastine and serpentine showed high activity at days 8 and 21 in treated leaves, causing the increase in anhydrovinblastine and serpentine.
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