Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Beyond complementation. Map-based cloning in Chlamydomonas reinhardtii.

Linda A Rymarquis1, Jocelyn M Handley, Mabel Thomas

  • 1Boyce Thompson Institute, Cornell University, Ithaca, New York 14853, USA.

Plant Physiology
|January 25, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The GT1 domain of RNase J ensures RNA quality control through dsRNA binding in Arabidopsis plastids.

Nucleic acids research·2026
Same author

Delivery mode impacts gut bacteriophage colonization during infancy.

Gut microbes reports·2025
Same author

The α form of Rubisco activase supports photosynthesis during heat stress in the absence of the β form in Setaria viridis.

Journal of experimental botany·2025
Same author

Hybridization capture sequencing for <i>Vibrio</i> spp. and associated virulence factors.

mBio·2025
Same author

Novel Toxin Biosynthetic Gene Cluster in Harmful Algal Bloom-Causing Heteroscytonema crispum: Insights into the Origins of Paralytic Shellfish Toxins.

Genome biology and evolution·2024
Same author

Revolutionizing academic hiring: a faculty cluster hire emphasizing teamwork.

Trends in plant science·2024

Map-based cloning in Chlamydomonas reinhardtii is advancing rapidly. New tools and genomic resources are accelerating the identification of gene mutations underlying photosynthetic phenotypes.

Area of Science:

  • Plant Biology
  • Genetics
  • Photosynthesis Research

Background:

  • Chlamydomonas reinhardtii is a key model organism for plant biology due to its genetic tractability and transformable genomes.
  • Identifying specific gene mutations from observed mutant phenotypes, particularly in photosynthesis, has been a significant challenge.

Purpose of the Study:

  • To discuss advancements in map-based cloning techniques for Chlamydomonas reinhardtii.
  • To highlight the application of new tools for faster gene identification from mutant phenotypes.

Main Methods:

  • Utilizing a molecular map with 506 markers and a draft nuclear genome sequence.
  • Implementing techniques such as bulked segregant analysis and marker duplexing.
  • Developing map-based cloning strategies using specific mutant strains (mcd4, mcd5).

Related Experiment Videos

Main Results:

  • Map-based cloning is becoming increasingly feasible in Chlamydomonas.
  • New tools are enhancing the speed of identifying causative genes for mutant phenotypes.
  • Recessive nuclear suppressors restoring photosynthesis in chloroplast mutants have been studied.

Conclusions:

  • Advances in genomic resources and molecular tools are revolutionizing gene discovery in Chlamydomonas.
  • Future developments in the Chlamydomonas genome initiative will further enhance map-based cloning capabilities.
  • These tools address limitations of traditional complementation methods for gene cloning.