Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.5K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.5K
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

998
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
998
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

7.5K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
7.5K
Types of Building Separation Joints01:23

Types of Building Separation Joints

601
Building separation joints divide large or complex building structures into smaller, discrete units that can move independently. These joints are categorized into three types: volume-change joints, settlement joints, and seismic separation joints.
Volume-change joints address the effects of expansion and contraction due to temperature and moisture variations. They are strategically placed at discontinuities in a building's mass where cracking is most likely and are spaced about 150 to 200...
601
The DNA Replication Fork01:02

The DNA Replication Fork

40.9K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
40.9K
Trends in Lattice Energy: Ion Size and Charge02:54

Trends in Lattice Energy: Ion Size and Charge

26.7K
An ionic compound is stable because of the electrostatic attraction between its positive and negative ions. The lattice energy of a compound is a measure of the strength of this attraction. The lattice energy (ΔHlattice) of an ionic compound is defined as the energy required to separate one mole of the solid into its component gaseous ions. For the ionic solid sodium chloride, the lattice energy is the enthalpy change of the process:
26.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Online affinity micro free-flow electrophoresis for the continuous monitoring of insulin <i>via</i> a competitive immunoassay.

Lab on a chip·2026
Same author

Identifying and minimizing primary sources of temporal broadening in online affinity micro free-flow electrophoresis.

The Analyst·2025
Same author

Assessing Surface Adsorption in Cyclic Olefin Copolymer Microfluidic Devices Using Two-Dimensional Nano Liquid Chromatography-Micro Free Flow Electrophoresis Separations.

Analytical chemistry·2023
Same author

Fabrication of µFFE Devices in COC via Hot Embossing with a 3D-Printed Master Mold.

Micromachines·2023
Same author

Reduced surface adsorption in 3D printed acrylonitrile butadiene styrene micro free-flow electrophoresis devices.

Electrophoresis·2019
Same author

Cytoplasmic nucleic acid-based XNAs directly enhance live cardiac cell function by a Ca<sup>2+</sup> cycling-independent mechanism via the sarcomere.

Journal of molecular and cellular cardiology·2019
Same journal

Correction: Regeneration-on-a-chip: a planarian microfluidic device enabling automated cultivation, individual tracking and <i>in vivo</i> imaging for regeneration study.

The Analyst·2026
Same journal

Optical photothermal infrared (O-PTIR) micro-spectroscopic characterisation of breast cancer cell lines: a comparative molecular profile of MCF7, MDA-MB-231, and SKBR3 cells.

The Analyst·2026
Same journal

A novel electrochemiluminescence sensor using Lu-Au@Ni-MOF for ultra-sensitive detection of permethrin.

The Analyst·2026
Same journal

Sensitive detection of meat spoilage VOCs and visual monitoring of freshness <i>via</i> a UiO-66-NH<sub>2</sub>@MR nanocomposite-based colorimetric paper sensor.

The Analyst·2026
Same journal

AuNPs/rGO-enhanced molecularly imprinted field-effect transistor sensor for highly selective detection of lactic acid in sweat.

The Analyst·2026
Same journal

Surface-engineered biochar-Cu-BTC hybrid for ultrasensitive dual detection of uric acid and nitrite.

The Analyst·2026
See all related articles

Related Experiment Video

Updated: Jan 28, 2026

Spatial Separation of Molecular Conformers and Clusters
10:37

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

11.8K

SELEX: just another separation?

Michael T Bowser1

  • 1University of Minnesota, Department of Chemistry, 207 Pleasant St. SE, Minneapolis, MN 55455, USA. bowser@chem.umn.edu

The Analyst
|January 25, 2005
PubMed
Summary
This summary is machine-generated.

Systematic Evolution of Ligands by Exponential Enrichment (SELEX) offers potential for ligand isolation but faces challenges. Recent advancements are improving aptamer affinity, stability, and throughput, revitalizing SELEX for diverse applications.

More Related Videos

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme
08:36

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

Published on: April 12, 2013

11.5K
Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

35.2K

Related Experiment Videos

Last Updated: Jan 28, 2026

Spatial Separation of Molecular Conformers and Clusters
10:37

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

11.8K
Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme
08:36

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

Published on: April 12, 2013

11.5K
Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

35.2K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Chemical Biology

Background:

  • Systematic Evolution of Ligands by Exponential Enrichment (SELEX) technology was initially promising for rapid isolation of high-affinity ligands.
  • Despite its potential, SELEX has faced limitations in aptamer affinity, stability, and experimental throughput, hindering widespread adoption.
  • Fifteen years post-discovery, SELEX is experiencing a resurgence due to ongoing research addressing these critical challenges.

Purpose of the Study:

  • To highlight the renewed interest and progress in SELEX technology.
  • To underscore the potential of SELEX in various scientific domains.
  • To discuss the overcoming of previous limitations in aptamer development.

Main Methods:

  • The abstract does not detail specific experimental methods.
  • It refers to ongoing research and recent advancements in SELEX protocols.
  • Focus is on the conceptual evolution and application of the SELEX technique.

Main Results:

  • SELEX technology is regaining prominence as a significant research topic.
  • Improvements in aptamer characteristics (affinity, stability) are being achieved.
  • The scope of SELEX applications is expanding across multiple disciplines.

Conclusions:

  • SELEX technology is overcoming its initial limitations.
  • The technique is becoming increasingly viable for applications in drug discovery, chemical analysis, genomics, and proteomics.
  • SELEX is poised to become a more widely accepted and powerful tool in molecular biology and biotechnology.