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Resveratrol interference with the cell cycle protects human neuroblastoma SH-SY5Y cell from paclitaxel-induced

R Rigolio1, M Miloso, G Nicolini

  • 1Dipartimento di Neuroscienze e Tecnologie Biomediche, Università degli Studi di Milano-Bicocca, Via Cadore 48, 20052 Monza, Italy.

Neurochemistry International
|January 27, 2005
PubMed

Insights

Resveratrol prevents paclitaxel-induced apoptosis in human neuroblastoma cells by halting the cell cycle at the S-phase. This mechanism explains how resveratrol protects cells from the damaging effects of chemotherapy.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Pharmacology

Background:

  • Resveratrol exhibits antiapoptotic properties against paclitaxel in human neuroblastoma (HN) SH-SY5Y cells.
  • Previous studies indicated resveratrol inhibits paclitaxel-induced apoptotic pathways.

Purpose of the Study:

  • To evaluate the antiapoptotic effect of resveratrol by examining its impact on cell cycle progression.
  • To elucidate the molecular mechanisms underlying resveratrol's protective action.

Main Methods:

  • Determined mitotic index in cultures treated with resveratrol and paclitaxel.
  • Analyzed cell cycle distribution using flow cytometry (FACS).
  • Assessed the modulation of key cell cycle regulatory proteins.

Main Results:

  • Resveratrol induced an S-phase cell cycle arrest in SH-SY5Y cells.
  • This arrest was linked to increased cyclin E and cyclin A, and decreased cyclin D1.
  • No alterations were observed in cyclin B1 and cdk 1 activation.

Conclusions:

  • Resveratrol's S-phase block prevents cells from entering mitosis, where paclitaxel is most effective.
  • This cell cycle interference explains resveratrol's observed antiapoptotic effect against paclitaxel treatment.

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