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Updated: Aug 19, 2026
Studying the Effects of Inhaled Environmental Pollutants on Olfactory Function in Mice
Published on: September 13, 2024
Olfactory epithelium destruction by ZnSO4 modified sulfhydryl oxidase expression in mice
Karine Bon1, Pascale Adami, Frédéric Esnard
1Laboratoire de Neurosciences, EA 481, IFR133, Université de Franche-Comté, UFR Sciences et Techniques, 1 Place Maréchal Leclerc, 25030 Besançon cedex, France.
Abstract:
Experimental destruction of olfactory neurons stimulates proliferation and differentiation of local neural precursors and is used as a model to study in vivo mechanisms for degeneration and regeneration of the nervous system. Quiescin-sulfhydryl oxidases (QSOX) have a potential role in the control of the cell cycle or growth regulation and have recently been described in the central nervous system. In mice, we show an expression of QSOX in olfactory mucosa. Northern- and western-blot analysis show that the destruction of olfactory epithelium is associated with a reversible reduction in QSOX expression. Interestingly, QSOX is not localized in olfactory neurons (ON) but in cells of the lamina propria, suggesting that olfactory epithelium destruction may act as a signal of down-regulation of QSOX expression.
Insights
Destruction of olfactory neurons in mice reduces quiescin-sulfhydryl oxidase (QSOX) expression in the olfactory mucosa. This suggests QSOX may play a role in nervous system regeneration following injury.
Area of Science:
- Neuroscience
- Cell Biology
- Regenerative Medicine
Background:
- Olfactory neuron destruction is a model for studying nervous system degeneration and regeneration.
- Quiescin-sulfhydryl oxidases (QSOX) are implicated in cell cycle control and growth regulation.
- QSOX has been identified in the central nervous system.
Purpose of the Study:
- To investigate the expression and regulation of QSOX in the mouse olfactory mucosa.
- To determine the role of QSOX in the context of olfactory epithelium regeneration.
Main Methods:
- Northern blot analysis to assess QSOX gene expression.
- Western blot analysis to evaluate QSOX protein levels.
- Experimental destruction of olfactory neurons in mice.
Main Results:
- QSOX is expressed in the olfactory mucosa of mice.
- Olfactory epithelium destruction led to a reversible decrease in QSOX expression.
- QSOX was localized to lamina propria cells, not olfactory neurons.
Conclusions:
- Olfactory epithelium destruction down-regulates QSOX expression.
- QSOX may be involved in the regenerative processes of the olfactory system.
- The findings suggest QSOX's role in nervous system repair mechanisms.
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