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Updated: Jan 31, 2026

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
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Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Published on: March 20, 2020

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ATG9A Is Overexpressed in Triple Negative Breast Cancer and Its In Vitro Extinction Leads to the Inhibition of

Aurore Claude-Taupin1, Leïla Fonderflick2, Thierry Gauthier3

  • 1INSERM, EFS BFC, UMR1098, Interactions Hôte-Greffon-Tumeur/Ingénierie Cellulaire et Génique, University Bourgogne Franche-Comté F-25000 Besançon, France. taupin.aurore@gmail.com.

Cells
|December 20, 2018
PubMed

Insights

Triple negative breast cancer (TNBC) lacks targeted therapies. This study identifies ATG9A as upregulated in TNBC and demonstrates its inhibition reduces cancer cell features, suggesting ATG9A as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Breast cancer (BC) mortality has decreased due to early detection and targeted treatments.
  • Triple negative breast cancer (TNBC) remains a challenge, lacking efficient targeted therapies.
  • The autophagy pathway is being explored for cancer treatment strategies.

Purpose of the Study:

  • To investigate the role of autophagy genes in breast cancer, particularly TNBC.
  • To identify potential new therapeutic targets for TNBC.
  • To analyze the expression of six autophagy genes in breast cancer tissues.

Main Methods:

  • Compared expression of six autophagy genes (ATG9A, ATG9B, BECLIN1, LC3B, NIX, P62/SQSTM1) in breast cancer and adjacent healthy tissues.
  • Validated ATG9A mRNA expression increase at the protein level in paraffin-embedded tissues.
  • Utilized shRNA and CRISPR/Cas9 to inhibit ATG9A expression in MDA-MB-436 TNBC cell line.

Main Results:

  • Observed increased ATG9A mRNA expression in TNBC samples.
  • Confirmed elevated ATG9A protein levels in TNBC tissues.
  • Demonstrated that ATG9A inhibition reduced in vitro cancer cell features.

Conclusions:

  • ATG9A is upregulated in triple negative breast cancer.
  • ATG9A plays a role in regulating cancer phenotypes.
  • ATG9A represents a potential novel biomarker and therapeutic target for TNBC.
Keywords:
ATG9ACRISPR/Cas9MDA-MB-436autophagyshRNAtriple negative breast cancer

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