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Related Experiment Videos

On-chip cell lysis by local hydroxide generation.

Dino Di Carlo1, Cristian Ionescu-Zanetti, Yolanda Zhang

  • 1Department of Bioengineering, University of California at Berkeley, Berkeley, CA, USA.

Lab on a Chip
|January 27, 2005
PubMed
Summary
This summary is machine-generated.

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This study introduces a new on-chip cell lysis technique using electro-generated hydroxide ions. This method efficiently lyses various cell types and neutralizes the lysate without a wash step, simplifying sample preparation.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Microfluidics

Background:

  • Cell lysis is a critical step in biological sample preparation.
  • Existing lysis methods can be time-consuming and require multiple steps.
  • On-chip methods offer potential for miniaturization and automation.

Purpose of the Study:

  • To develop and validate a novel on-chip cell lysis method.
  • To investigate the mechanism of cell membrane poration by electro-generated hydroxide.
  • To demonstrate the efficiency of the method across different cell types and in flow-through systems.

Main Methods:

  • On-chip local hydroxide electro-generation for cell membrane poration.
  • Finite element simulations to predict hydroxide concentration.

Related Experiment Videos

  • Monitoring cytoplasmic molecule release using membrane-impermeant dyes.
  • Integration of a mechanical filter for flow-through lysis.

    • Main Results:

    • Effective lysis of red blood cells, HeLa, and Chinese Hamster Ovary (CHO) cells.
    • Demonstrated release of cytoplasmic molecules from lysed cells.
    • Lysis rates correlated with hydroxide concentration and distance from the cathode.
    • Lysis efficiency found to be proportional to hydroxide concentration in flow-through experiments.

    Conclusions:

    • The developed on-chip electro-generation method provides efficient cell lysis.
    • The integrated system neutralizes lysate pH, eliminating the need for a wash step.
    • This technique is suitable for various cell types and adaptable for flow-through applications.