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Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
Published on: January 22, 2014
Engineering and expression of a recombinant fusion protein possessing fibroblast growth factor-2 and fibronectin
Jun-Hyeog Jang1, Chong-Pyoung Chung
1Department of Biochemistry, Inha University College of Medicine, Jung Gu, Incheon 400-712, Korea. juhjang@inha.ac.kr
Abstract:
There is a synergistic effect between fibronectin and fibroblast growth factor 2 (FGF-2) on osteoblast cell adhesion through an extracellular, signal-regulated kinase pathway. Here we describe the engineering of a fusion protein containing fibronectin fragment (FNIII9-10) connected to the COOH-terminus of FGF-2. Purified FGF2-FNIII9-10 fusion protein exhibited a significant increase of cell adhesion and proliferation of MG63 cells compared with FNIII9-10 alone (p < 0.05).
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