Related Experiment Video
Updated: Aug 19, 2026

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
Published on: April 5, 2017
Simple fibroblast-based assay to test the pyrazinamide susceptibility of Mycobacterium tuberculosis
Takemasa Takii1, Sonomi Hamasaki, Kazue Hirano
1Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-Dori, Mizuho-Ku, Nagoya 467-8603, Japan. ttakii@phar.nagoya-cu.ac.jp
Abstract:
A simple fibroblast-based assay (SFA) was found to be efficient in evaluating the susceptibilities of clinical isolates of Mycobacterium tuberculosis to pyrazinamide (PZA). Forty-five clinical isolates were examined. The MICs of PZA for susceptible strains in an SFA were between 3.13 and 12.5 microg/ml, and the MICs of PZA for resistant strains were more than 100 microg/ml.
