Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Improved gene expression by a U3-based retroviral vector.

B F Chen1, C L Hsieh, D S Chen

  • 1Hepatitis Research Center, National Taiwan University Hospital, R.O.C.

Biochemical and Biophysical Research Communications
|April 15, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Connecting the spherical harmonics with the periodic orbits: application to optical microsphere cavities.

Optics express·2026
Same author

Effects of thermal cycling and room-temperature ageing on bismuth precipitates in Sn-Ag-Cu-Bi solder joints.

Journal of materials science·2026
Same author

Effects of bismuth on the microstructure and crack propagation in Sn-Ag-Cu-Bi solder joints during thermal cycling.

Journal of materials science. Materials in electronics·2025
Same author

Corrigendum to "Proteomic characterization of outer membrane vesicles from gut mucosa-derived fusobacterium nucleatum". J Proteomics. 2019 Jan 8;195:125-137.

Journal of proteomics·2019
Same author

A luminescence-based assay for evaluating bactericidal antibody to Borrelia burgdorferi in vaccinated horses' serum.

Equine veterinary journal·2019
Same author

Association between HLA-DRB1 alleles and tuberculosis: a meta-analysis.

Genetics and molecular research : GMR·2015
Same journal

Biosynthesis, characterization and biological potential of microbe-mediated silver nanoparticles using thermophilic actinomycetes, Streptomyces nigra.

Biochemical and biophysical research communications·2026
Same journal

COP9 signalosome 8 mediated autophagy drives proliferation, invasion, and metastasis in pancreatic ductal adenocarcinoma.

Biochemical and biophysical research communications·2026
Same journal

Tumor budding in colorectal cancer: partial EMT, microenvironmental remodeling, and metastatic competence.

Biochemical and biophysical research communications·2026
Same journal

Exploring the therapeutic versatility and multitarget pharmacological potential of acyl hydrazone-hydrazide scaffolds.

Biochemical and biophysical research communications·2026
Same journal

The plasma membrane H<sup>+</sup>-ATPase OSA2 negatively regulates salt tolerance in rice seedlings.

Biochemical and biophysical research communications·2026
Same journal

MiR-425-5p modulation of CREB1 affects inflammatory response and motor recovery after spinal cord injury.

Biochemical and biophysical research communications·2026
See all related articles

New U3-based retroviral vectors significantly enhance gene expression compared to conventional vectors. This improved gene delivery method benefits various promoters, offering a more advantageous approach for genetic engineering applications.

Area of Science:

  • Molecular Biology
  • Gene Therapy
  • Retroviral Vector Technology

Background:

  • Conventional retroviral vectors face limitations in achieving optimal transgene expression.
  • Gene expression levels are often influenced by vector design and promoter integration sites.

Purpose of the Study:

  • To develop and evaluate a novel U3-based retroviral vector for enhanced gene expression.
  • To compare the efficacy of U3-based vectors against conventional retroviral vectors.

Main Methods:

  • Construction of a U3-based retroviral vector with gene insertion at the U3 region of the 3' long terminal repeats (LTR).
  • Evaluation of gene expression using cytomegalovirus immediate early gene promoter (CMV), SV40 early gene promoter (SV), and herpes simplex virus thymidine kinase gene promoter (TK).

Related Experiment Videos

  • Comparison of expression levels between U3-based and conventional retroviral vectors.

    • Main Results:

    • U3-based vectors significantly enhanced the expression of transduced genes across all tested promoters (CMV, SV, TK).
    • The potency of SV and TK promoters, previously suppressed in conventional vectors, was restored in U3-based vectors.
    • Gene duplication and transfer to the 5'-LTR in infected cells contributed to enhanced expression.

    Conclusions:

    • U3-based retroviral vectors offer a significant advantage over conventional vectors for improving gene expression.
    • This vector design overcomes limitations associated with promoter suppression in traditional retroviral systems.
    • The findings support the utility of U3-based vectors for advanced gene therapy and genetic engineering applications.