Albumin as a marker of plasma transudation in experimental skin lesions

Liquid chromatography measurements of albumin levels were obtained for experimental incision wounds, excoriations, and heat and freezing injuries of different ages. Hemoglobin levels in tissue specimens were measured and an equivalent amount of blood-related albumin was subtracted from the analysis results. In specimens taken immediately after death, the mean albumin level as compared to control skin was increased by about 2-fold in freezing injuries aged 60 min. In all other lesions, the same increase was observed even after 30 min. The mean albumin level was about 3-fold as compared with the control skin in excoriations aged 30 min, heat and freezing injuries aged 4 h, and incision wounds aged 12 h. An approximately 5-fold increase was seen in heat and freezing injuries aged 1 and 2 weeks. A marked decrease occurred in mean albumin levels in all lesions aged 4 weeks. An increase in albumin in wounds and excoriations was demonstrable also in specimens taken 3 days postmortem. Postmortem hypostasis resulted in a 1.1 to 1.4-fold increase in mean albumin levels in wounds and excoriations inflicted 1 min postmortem.