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Absolute determination method using chirality for glufosinate and bialaphos by gamma-cyclodextrin modified capillary
Takayuki Asami1, Hisanori Imura, Akira Ohashi
1Department of Environmental Sciences, Faculty of Science, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki 310-8512, Japan.
Summary
A new chiral method accurately determines glufosinate and bialaphos herbicides without standards. This technique measures enantiomeric ratios, offering precise quantification for agricultural chemical analysis.
Area of Science:
- Analytical Chemistry
- Environmental Science
- Agricultural Chemistry
Background:
- Accurate quantification of herbicides like glufosinate (GLUF) and bialaphos (BIAL) is crucial for agricultural applications and environmental safety.
- Existing methods often rely on calibration curves or comparison standards, which can be resource-intensive and prone to errors.
- Chirality plays a significant role in the biological activity and environmental fate of these herbicides.
Purpose of the Study:
- To develop a novel, calibration-free absolute determination method for chiral herbicides.
- To establish a method based on enantiomeric ratio changes for quantifying glufosinate (D,L-GLUF) and bialaphos (BIAL).
- To validate the proposed method's accuracy, precision, and applicability to commercial herbicide formulations.
Main Methods:
- A novel chiral determination approach utilizing enantiomeric ratio shifts after spiking samples with known enantiomer amounts.
- Quantification of D,L-GLUF via derivatization with dansyl chloride and analysis using gamma-cyclodextrin modified capillary zone electrophoresis.
- Determination of BIAL by quantifying the GLUF produced after acid hydrolysis.
Main Results:
- The method achieved high accuracy for D- and L-GLUF, with mean values agreeing within 1.6% of expected values.
- Excellent reproducibility was demonstrated, with a standard deviation of less than 2.8% for the enantiomeric ratio measurements.
- The method was successfully applied to analyze commercial herbicide products, confirming its practical utility.
Conclusions:
- A novel, reliable, and calibration-free chiral method for determining glufosinate and bialaphos has been successfully developed.
- The proposed technique offers a significant advancement in the analysis of chiral herbicides, reducing the need for external standards.
- This method provides a valid and useful tool for the quality control and analysis of commercial herbicide formulations.