Effects of D-myo-inositol 1-phosphate on the transfer function of phosphatidylinositol transfer protein alpha
Hiroaki Komatsu1, Jan Westerman, Gerry T Snoek
1Department of Pathology, Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Abstract:
The lipid metabolite D-myo-inositol-1-phosphate is shown to increase the phospholipid transfer activity of phosphatidylinositol transfer protein alpha from liposomal and liver microsomal membranes. Dose-response curves indicated substantial enhancements of transfer in the low mM range that upon normalization were independent of membrane composition or the identity of the transferred phospholipid. The unnormalized effect is potentiated by anionic membrane surface charge and substantial membrane phosphatidylethanolamine content consistent with alterations of the protein's membrane binding affinity and alterations of surface electrostatic interactions as contributing factors.
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