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Updated: Aug 19, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
Published on: December 4, 2021
Serially coupled capillary columns supercritical fluid chromatography with midpoint pressure control
Yukio Hirata1, Katsuhito Tsuda, Etsuro Imamura
1School of Materials Science, Toyohashi University of Technology, Toyohashi 441-8580, Japan. hirata@chrom.tutms.tut.ac.jp
Abstract:
Two capillary columns of different polarities were coupled in series by means of a coupling restrictor. The pressure of the first column and the midpoint pressure (between the coupling restrictor and the second column) were controlled independently of each other using two pumps. The selectivity of this separation system was highly dependent on the pressure difference and could be continuously changed between those of two columns. The pressure difference could be changed even in course of separation for fine tuning of the selectivity. Several examples were shown to demonstrate the utility of this method.
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