Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Small CTD phosphatases function in silencing neuronal gene expression.

Michele Yeo1, Soo-Kyung Lee, Bora Lee

  • 1Department of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.

Science (New York, N.Y.)
|February 1, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevalence of Avoidant/Restrictive Food Intake Disorder (ARFID) in children with and without food allergy.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2026
Same author

Development of a rapid and simple Xeno Nucleic Acid (XNA) sensor-based microRNA detection platform for Parkinson's disease diagnostics.

Journal of nanobiotechnology·2026
Same author

Refined obesity, smoking exposure, and lipid metrics in mortality risk assessment: a nationwide cohort analysis.

PloS one·2026
Same author

<i>Undaria pinnatifida</i> intake improves lipid metabolism in older women during marine healing: a nuclear magnetic resonance-based metabolomics analysis.

Nutrition research and practice·2026
Same author

Circuit organization and transcriptomic heterogeneity of sympathetic circuits innervating cranial structures.

Cell reports·2026
Same author

Dehydration-induced condensation of AGO1 modulates miRNA functionality.

The Plant cell·2026

Small CTD phosphatases (SCPs) are recruited by REST/NRSF to repress neuronal gene transcription in non-neuronal cells. SCP activity is crucial for silencing neuronal genes and is evolutionarily conserved.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Cellular Neuroscience

Background:

  • Neuronal gene transcription is silenced in non-neuronal cells by the REST/NRSF complex.
  • The mechanism of this silencing, particularly the role of phosphatases, remains incompletely understood.

Purpose of the Study:

  • To investigate the role of small CTD phosphatases (SCPs) in REST/NRSF-mediated neuronal gene silencing.
  • To elucidate the mechanism by which SCPs contribute to transcriptional repression.

Main Methods:

  • Examined the function of SCPs 1-3 in mammalian cells.
  • Utilized phosphatase-inactive SCP mutants.
  • Employed small interfering RNA (siRNA) in Drosophila S2 cells.
  • Assessed neuronal gene expression and differentiation in P19 stem cells.

Related Experiment Videos

Main Results:

  • REST/NRSF recruits SCPs to neuronal genes containing RE-1 elements.
  • SCPs mediate neuronal gene silencing in non-neuronal cells.
  • Inactive SCPs disrupt REST/NRSF function and promote neuronal differentiation.
  • Drosophila SCP depletion leads to unmasked neuronal gene expression.

Conclusions:

  • SCP activity is a key, evolutionarily conserved regulator of neuronal gene silencing.
  • SCPs act globally to repress neuronal gene expression in non-neuronal cell types.
  • This mechanism is vital for maintaining cell-type-specific gene expression patterns.