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Introduction of tyramide signal amplification (TSA) to pre-embedding nanogold-silver staining at the electron

Seung-won Lee1, Song Eun Lee, Seong Hyuk Ko

  • 1Department of Anatomy, Chonnam National University Medical School, Gwangju, Korea. seunglee@chonnam.ac.kr

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|February 3, 2005
PubMed

The tyramide signal amplification (TSA) technique has been shown to detect scarce tissue antigens in light and electron microscopy. In this study we applied the TSA technique at the electron microscopic level to pre-embedding immunocytochemistry. This protocol was compared to the non-amplified protocol. With the TSA protocol, the labeling of GM130, a cis-Golgi matrix protein, was tested in a cell line and found to be highly sensitive and more enhanced than that with the simple protocol. Moreover, the gold particles were well localized to the cis-side of the Golgi apparatus in both the TSA and the simple protocol.

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