Related Experiment Video
Updated: Jan 25, 2026

Author Spotlight: Advancing Anaerobic Microbiota Research Using a Novel Respirometry Protocol
Published on: April 26, 2024
Simultaneous determination of activated sludge floc size distribution by different techniques
R Govoreanu1, H Saveyn, P Van der Meeren
1BIOMATH, Dept Applied Mathematics, Biometrics and Process Control, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium. ruxandra@biomath.UGent.be
Comparing activated sludge floc size distribution (FSD) measurement techniques reveals that MastersizerS and SHAPE methods provide similar results. Automated image analysis (IMAN) overestimates floc size, while time of transition (TOT) shows bimodal distributions.
Area of Science:
- Environmental Engineering
- Water Treatment Technologies
- Wastewater Microbiology
Background:
- Activated sludge flocs are crucial for wastewater treatment efficiency.
- Understanding floc size distribution (FSD) is vital for process optimization.
- Variability in FSD measurements can hinder accurate process assessment.
Purpose of the Study:
- To compare different techniques for measuring activated sludge floc size distribution (FSD).
- To identify the limitations and agreement between various measurement methods.
- To evaluate the impact of measurement principles on FSD data.
Main Methods:
- Utilized three instruments: MastersizerS, automated image analysis (IMAN), and CIS-100 (with TOT and SHAPE channels).
- Collected activated sludge samples from a pilot-scale sequencing batch reactor (SBR).
- Flocculated samples to steady state to minimize measurement variability.
Main Results:
- MastersizerS and SHAPE (image analysis) yielded similar volume-weighted FSD.
- IMAN overestimated floc size; TOT often showed bimodal distributions.
- Number distributions from TOT and SHAPE agreed, while MastersizerS showed submicron sizes and IMAN larger sizes.
Conclusions:
- Measurement principle significantly impacts observed FSD, affecting data interpretation.
- MastersizerS and SHAPE are comparable for volume-weighted FSD.
- Careful consideration of technique limitations is essential for accurate activated sludge characterization.
More Related Videos
09:27Measuring the Shape and Size of Activated Sludge Particles Immobilized in Agar with an Open Source Software Pipeline
Published on: January 30, 2019
15:19Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
Related Concept Videos
Dose Size and Dosing Frequency: Determination Methods
Pore Size Distribution
Adequate...
Drug Distribution: Volume of Distribution
Cell Size
Surface Area
Cells can take in nutrients and water via diffusion through the plasma membrane itself or through specific channels in the membrane. The area of the membrane surrounding...
F Distribution
Volume of Distribution