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Updated: Aug 8, 2026

Microfluidic Mixers for Studying Protein Folding
Published on: April 10, 2012
Protein unfolding and refolding under force: methodologies for nanomechanics
Bruno Samorì1, G Zuccheri, R Baschieri
1Department of Biochemistry G. Moruzzi, University of Bologna, Italy. bruno.samori@unibo.it
Abstract:
An increasing number of inter- and intramolecular interactions can nowadays be probed using single-molecule manipulation techniques. Protein unfolding and refolding is the most representative--though complex--of these interactions. Herein, we review the main modes of performing a force unfolding experiment: the velocity clamp and the new force clamp mode. We also compare some of the physical aspects behind the two most frequently used single-molecule manipulation instrumentations: optical tweezers and atomic force microscopes.
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