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Updated: Jan 25, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
[Effect on synthesis of nitric oxide in myocardium by local cryoablation]
Bai-qin Zhao1, Jia-guang Zhu, Ming Zhang
1The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
Objective:
To study the effect on synthesis of nitric oxide in myocardium by local cryoablation and to investigate its mechanism.
Methods:
Myocardium was cryoablated locally by a probe cooled to -60 degrees C and rewarmed by normal salt solution, nitric oxide and its synthesis enzyme were measured before and after cryoablation. L-arginine or methylene blue was added before and during cryoablation and the effect of these drugs on synthesis of nitric oxide was studied.
Results:
Nitric oxide and its synthesis enzyme decreased after cryoablation; L-arginine preserved the synthesis of nitric oxide and methylene blue inhibited the synthesis of nitric oxide. However, nitric oxide in serum did not change.
Conclusion:
Nitric oxide and its synthesis enzyme in myocardium decrease after cryoablation.
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