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Related Experiment Videos

Comparing and combining predictors of mostly disordered proteins.

Christopher J Oldfield1, Yugong Cheng, Marc S Cortese

  • 1Molecular Kinetics, Inc., 6201 La Pas Trail, Suite 160, Indianapolis, Indiana 46268, USA.

Biochemistry
|February 9, 2005
PubMed
Summary
This summary is machine-generated.

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Intrinsically disordered proteins (IDPs) are crucial for cell function. A new consensus method reveals a high frequency of IDPs in eukaryotes, impacting protein structure and function studies.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genomics

Background:

  • Intrinsically disordered proteins (IDPs) and regions perform diverse cellular functions.
  • Eukaryotic cells frequently contain proteins with disordered regions, with some being predominantly disordered.
  • Existing prediction methods for disordered proteins include charge-hydropathy and disorder score distributions.

Purpose of the Study:

  • To reexamine and compare existing prediction methods for disordered proteins.
  • To introduce a novel consensus-based prediction method for identifying disordered proteins.
  • To assess the prevalence of mostly disordered proteins across various genomes.

Main Methods:

  • Reexamination of net charge-hydropathy distribution and disorder prediction score distribution methods.

Related Experiment Videos

  • Development and application of a new consensus prediction method.
  • Genome-wide analysis of protein disorder in selected prokaryotic and eukaryotic species.

    • Main Results:

    • Comparison of prediction accuracies between existing methods.
    • Identification of a significant proportion of mostly disordered proteins in various organisms.
    • Quantification of mostly disordered proteins: 4.5% in Yersinia pestis, 5% in Escherichia coli K12, 6% in Archaeoglobus fulgidus, 8% in Methanobacterium thermoautotrophicum, 23% in Arabidopsis thaliana, and 28% in Mus musculus.

    Conclusions:

    • The consensus method provides a robust approach for predicting mostly disordered proteins.
    • Eukaryotes exhibit a surprisingly high frequency of mostly disordered proteins.
    • The prevalence of disordered proteins has significant implications for structural biology and functional genomics projects.