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Related Experiment Videos

A new insertion sequence, IS14999, from Corynebacterium glutamicum.

Yota Tsuge1,2, Kana Ninomiya2, Nobuaki Suzuki2

  • 1Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST), Ikoma, Nara 630-0101, Japan.

Microbiology (Reading, England)
|February 9, 2005
PubMed
Summary
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A novel insertion sequence, IS14999, was identified in Corynebacterium glutamicum. This element represents a new subfamily within the IS630/Tc1 superfamily, potentially acquired via lateral gene transfer and useful for genetic engineering.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Insertion sequences (IS) are mobile genetic elements found in prokaryotes.
  • The IS630/Tc1 superfamily is a diverse group of IS elements.
  • Corynebacterium glutamicum is a bacterium with significant industrial applications.

Purpose of the Study:

  • To isolate and characterize a new insertion sequence from Corynebacterium glutamicum ATCC 14999.
  • To determine the phylogenetic relationship and potential origin of the novel IS element.
  • To assess the potential utility of the new IS element for genetic manipulation.

Main Methods:

  • Nucleotide sequencing and analysis of the IS element.
  • Open reading frame prediction and amino acid sequence homology searches.

Related Experiment Videos

  • Phylogenetic analysis of transposase amino acid sequences.
  • Insertion site analysis in Corynebacterium glutamicum.

    • Main Results:

    • A new 1149 bp IS element, IS14999, was identified with imperfect terminal inverted repeats.
    • IS14999 encodes a putative transposase with homology to the IS630/Tc1 superfamily, featuring a unique DDE motif spacing.
    • Phylogenetic analysis suggests IS14999 is more closely related to eukaryotic Tc1/mariner elements, indicating possible eukaryotic origin via lateral gene transfer.
    • IS14999 exhibits random transposition with 5'-TA-3' dinucleotide target duplication, characteristic of the IS630/Tc1 family.

    Conclusions:

    • IS14999 represents a novel subfamily within the IS630/Tc1 superfamily.
    • Its phylogenetic placement suggests a recent lateral gene transfer event from eukaryotic sources.
    • IS14999 is a potentially valuable tool for genetic manipulation in corynebacteria and related species.