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Published on: April 23, 2012
Application of solid-phase extraction to quantitatively determine cyproconazole and tebuconazole in treated wood
Teruhisa Miyauchi1, Mitsunori Mori, Katsuhiko Ito
1Hokkaido Forest Products Research Institute, Nishikagura 1-10, Asahikawa 071-0198, Japan. miyauchi@fpri.asahikawa.hokkaido.jp
Abstract:
Solid-phase extraction (SPE) procedures were developed to avoid interference during the quantitative determination of cyproconazole and tebuconazole co-existing in wood extractives. Five species of wood were used, Japanese cedar (Cryptomeria japonica), Japanese larch (Larix leptolepis), Yezo spruce (Picea jezoensis), Sakhalin fir (Abies sachalinensis), and western hemlock (Tsuga heterophylla). Methanol extractives from the heartwood of all wood samples, except western hemlock, interfered with the quantitative determination of cyproconazole and tebuconazole using liquid chromatography (LC) with UV detection (LC-UV). SPE with Oasis MCX was effective in avoiding this interference. This method also reduced the time and volume of mobile phase required for LC-UV, since wood extractives with long retention times were also removed.
