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Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Published on: March 8, 2012
[Study on anti-HPV activity of Asarum heterotropoides]
Yuanhui Deng1, Yi Feng, Jing Sun
1Guangdong Hospital of TCM, Guangzhou 510120.
Objective:
To screen anti-HPV effective fraction from Asarum heterotropoides.
Methods:
Asarum heterotropoides was extracted with systematic solvents (petroleum benzine, ether, ethyl acetate, n-butanol, ethanol and distilled water) accordingly. The Polymerase Chain Reaction (FQ-PCR) was applied in the vitro pharmacodynamics for the extracts from Asarum heterotropoides. The amplification of HPV-DNA in the lesions of the isolated Condyloma acuminatum.
Results:
After the water extracts of Asarum heterotropoides was used for HPV-DNA, the PCR amplification showed negative, with the minimum effective concentration 0.4 g/ml. But the other solvents extracts from Asarum heterotropoides appeared positive.
Conclusion:
The water extracts from Asarum heterotropoides are effective on anti-Human papillomavirus.
