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Updated: Aug 19, 2026
Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis
Published on: September 28, 2018
Analysis of outer membrane protein complexes and heat-modifiable proteins in Neisseria strains using two-dimensional
Sandra Sánchez1, Jesús Arenas, Ana Abel
1Departamento de Microbiología, Facultad de Farmacia, Universidad de Santiago, 15782 Santiago de Compostela, Spain.
Abstract:
Two-dimensional diagonal SDS-PAGE was used to resolve membrane complexes and identify proteins with temperature-dependent mobility in Neisseria meningitidis and N. lactamica. The main membrane complexes were composed of porins and were formed by heteromers of PorA, PorB and RmpM in N. meningitidis, and by PorB and RmpM in N. lactamica. Also, other proteins, including Opa, with temperature-dependent mobility were clearly demonstrated. The method allows improved detection of the components of membrane complexes and proteins with temperature-dependent mobility which is difficult to resolve with other analytical approaches.
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