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DNA fingerprinting in cattle using the probe pV47.
G Dolf1, M L Glowatzki, C Gaillard
1Institute of Animal Breeding, University of Berne, Switzerland.
Animal Genetics
|January 1, 1992
Summary
This study used multilocus probes to analyze cattle DNA fingerprints. The results show high band sharing in specific Swiss cattle populations, confirming parentage in offspring.
Area of Science:
- Genetics
- Animal Science
- Molecular Biology
Background:
- DNA fingerprinting is a powerful tool for animal genetics.
- Multilocus probes offer high discriminatory power for individual identification.
- Understanding genetic diversity and relatedness is crucial for livestock breeding programs.
Purpose of the Study:
- To characterize DNA fingerprints in cattle using the multilocus probe pV47.
- To estimate band sharing probabilities in different cattle populations.
- To validate the accuracy of DNA fingerprinting for parentage testing.
Main Methods:
- Detection of DNA fragments using the multilocus probe pV47 in cattle.
- Analysis of band patterns in various cattle groups, including different breeds and specific Swiss populations (Simmental, Brown Swiss).
- Calculation of band sharing probabilities and verification of inheritance in offspring.
Main Results:
- The pV47 probe detected an average of nine cattle DNA bands ranging from 23 kb to 4 kb.
- Band sharing probabilities varied: 33% in a mixed breed group (20 individuals, 12 breeds), 42% in Swiss Simmental (10 individuals), and 58% in Swiss Brown Swiss (11 individuals).
- DNA fingerprint analysis of 38 offspring (277 bands) confirmed that all bands could be traced to their parents, validating the method's accuracy.
Conclusions:
- The multilocus probe pV47 is effective for cattle DNA fingerprinting.
- Significant differences in band sharing exist between cattle populations, reflecting genetic structure.
- DNA fingerprinting using pV47 provides reliable parentage verification in cattle.