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Updated: Aug 19, 2026

Breeding by Design for Functional Rice with Genome Editing Technologies
Published on: January 3, 2025
Genetically modified rice seeds accumulating GLP-1 analogue stimulate insulin secretion from a mouse pancreatic
Koichi Sugita1, Saori Endo-Kasahara, Yoshifumi Tada
1Forestry Science Laboratory, Nippon Paper Industries Co., Ltd., 5-21-1, Oji, Kita-ku, Tokyo 114-0002, Japan.
Abstract:
Glucagon-like peptide-1 (7-36) amide (GLP-1) is the most potent physiological insulinotropic hormone in humans. We produced large amounts of a GLP-1 analogue, [Ser8, Gln26, Asp34]-GLP-1, which is resistant to trypsin-digestion, as part of a chimeric rice seed storage protein, a 26 kDa globulin, in genetically modified rice seeds. Junction sites between GLP-1 analogue and globulin were replaced by tryptic cleavage sites. The highest level of GLP-1 analogue accumulation was approximately 20-50 microg per seed. We found that GLP-1 analogue derived from trypsin-digested genetically modified rice seeds stimulated insulin secretion from a mouse pancreatic beta-cell line, MIN6.
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