Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Identifying protein-protein interactions in somatic hypermutation.

Myron F Goodman1, Matthew D Scharff

  • 1University of Southern California, Los Angeles, CA 90089, USA. mgoodman@usc.edu

The Journal of Experimental Medicine
|February 16, 2005
PubMed
Summary

Somatic hypermutation (SHM) generates antibody diversity for high-affinity binding. Pure biochemical approaches are poised to illuminate the mechanisms of SHM in antibody variable regions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct visualisation of post-replication gap formation at the bacterial RRS.

bioRxiv : the preprint server for biology·2026
Same author

Acinetobacter baumannii encodes multiple mutagenically and biochemically active DNA polymerase V variants.

Nucleic acids research·2026
Same author

Pathogen-encoded Rum DNA polymerase drives rapid bacterial drug resistance.

Nucleic acids research·2024
Same author

Modifying the Basicity of the dNTP Leaving Group Modulates Precatalytic Conformational Changes of DNA Polymerase β.

Biochemistry·2024
Same author

Controlling genome topology with sequences that trigger post-replication gap formation during replisome passage: the E. coli RRS elements.

Nucleic acids research·2024
Same author

Controlling Genome Topology with Sequences that Trigger Post-replication Gap Formation During Replisome Passage: The <i>E. coli</i> RRS Elements.

bioRxiv : the preprint server for biology·2023

Area of Science:

  • Immunology
  • Molecular Biology
  • Biochemistry

Background:

  • Somatic hypermutation (SHM) is crucial for developing high-affinity antibodies through immunoglobulin gene diversification.
  • Traditional research relied on cell and animal models, alongside human genetic studies, to understand SHM pathways.

Discussion:

  • Biochemical methods, historically underutilized, are now emerging as critical tools for dissecting SHM.
  • Understanding the precise molecular players and reactions involved in SHM is essential.

Key Insights:

  • The study highlights the impending shift towards biochemical strategies to investigate SHM.
  • This approach promises to reveal the detailed mechanisms of somatic mutation in antibody variable regions.

Outlook:

Related Experiment Videos

  • Biochemistry is expected to play a central role in elucidating the complete SHM pathway.
  • Future research will likely focus on in vitro reconstitution and analysis of SHM components.