[Study on the fingerprints of Belamcanda chinensis (L.) DC by capillary electrophoresis]

A capillary electrophoresis fingerprints (CEFP) method was established to evaluate the quality of Belamcanda chinensis (L.) DC. The background electrolyte (BGE) was a 80 mmol/L boric acid containing 15 mmol/L borate solution (adjusted pH 9.7 with KOH solution). The detection wavelength was 228 nm and 12.1 kV was applied. The Belamcanda chinensis (L.) DC was extracted by water to obtain a CEFP containing 21 peaks. The CEFP has good precision, reproducibility and stability with the relative standard deviations (RSDs) of the relative migration time less than 3.5% and the RSDs of the relative peak area about 5.0%. There were good similarities between the standard CEFP and each CEFP of samples collected from ten different places. The coefficients were from 0.913 to 0.993. Meanwhile, fundamental studies related to the methods in peak identification of fingerprints and content evaluation of different sample constituents were carried out.