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Updated: Aug 8, 2026

Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
A tandem mass spectrometric study of saccharides at high mass resolution
Raymond E March1, Christopher J Stadey
1Department of Chemistry, Trent University, Peterborough, ON, K9J 7B8, Canada. rmarch@trentu.ca
Abstract:
Nine monosaccharides and four disaccharides were mass analyzed using a quadrupole time-of-flight tandem mass spectrometer combined with an electrospray ionization source. Product ion mass spectra of deprotonated, protonated, and sodiated saccharides were observed and were compared within each group of saccharides. Each of the deprotonated pentoses, hexoses and disaccharides yielded a significantly different product ion mass spectrum with the exception of alpha-lactose and beta-lactose. The disaccharides alpha- and beta-lactose differ only at the glycosidic linkage. Product ion mass spectra of protonated and sodiated alpha- and beta-lactose were indistinguishable also.
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