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Updated: Aug 19, 2026

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
Published on: March 30, 2020
[The establishment and clinical application of multicolor fluorescence in situ hybridization]
Qing Li1, Xiao-fang Sun, Xin-jie Chen
1Guangzhou Second People's Hospital, Guangzhou Research Institute of Obstetrics and Gynecology, Guangzhou 510150, China. qinglicn@sina.com.cn
Objective:
To establish a rapid and convenient method of multi-color fluorescence in situ hybridization (FISH) on several different tissue samples: peripheral blood samples, amniotic fluid, embryos and bone marrow samples.
Methods:
FISH analysis was carried out on different tissue samples, using probes specific for chromosomes 13,18,21,X and Y or for BCR/ABL gene.
Results:
FISH analysis could reveal hybridization signals on metaphase chromosomes and interphase nuclei, it is also possible to detect chromosome aberrations.
Conclusion:
Multi-color FISH is clinically useful tool, which can be used as an adjunct to conventional chromosome analysis for prenatal diagnosis , preimplantation genetic diagnosis and diagnosis of leukemia.
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