Related Experiment Video

Updated: Aug 19, 2026

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography
07:32

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography

Published on: March 30, 2022

"Uncorking" of liposomes by matrix metalloproteinase-9

Nihar R Sarkar1, Theresa Rosendahl, Aaron B Krueger

  • 1Department of Chemistry and Molecular Biology, North Dakota State University, Fargo, North Dakota 58105, USA. sanku.mallik@ndsu.nodak.edu

Chemical Communications (Cambridge, England)
|February 19, 2005
PubMed

Insights

This study introduces a novel triggered release method for liposomes, utilizing the enzyme matrix metalloproteinase-9 (MMP-9). This approach enables precise release of liposomal contents in response to specific biological cues.

Area of Science:

  • Biochemistry
  • Materials Science
  • Drug Delivery

Background:

  • Liposomes are widely used drug delivery vehicles.
  • Controlled release of encapsulated substances is crucial for therapeutic efficacy.
  • Enzyme-responsive systems offer targeted drug release mechanisms.

Purpose of the Study:

  • To develop and describe a triggered release methodology for liposomal contents.
  • To investigate the use of matrix metalloproteinase-9 (MMP-9) as a trigger for liposomal release.

Main Methods:

  • Liposomes were engineered for MMP-9 sensitivity.
  • Enzyme-triggered release assays were performed.
  • Characterization of liposomal integrity and payload release was conducted.

Main Results:

  • Demonstrated successful triggered release of liposomal contents.
  • Confirmed MMP-9's efficacy in cleaving the liposomal structure.
  • Quantified the release kinetics in response to MMP-9.

Conclusions:

  • A novel MMP-9-triggered liposomal release system was successfully developed.
  • This methodology offers a promising strategy for targeted drug delivery.
  • Further research can explore applications in various therapeutic areas.

More Related Videos

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells10:07

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

Published on: January 7, 2019

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
Lipid Digestion01:06

Lipid Digestion

Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.