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Updated: Aug 19, 2026

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
Published on: April 1, 2022
Role of the Notch signalling pathway in tooth morphogenesis
Thimios A Mitsiadis1, Laure Regaudiat, Thomas Gridley
1Department of Craniofacial Development, Facial Genetics Laboratory, GKT Dental Institute, Kings College London, Floors 27-28 Guy's Tower, Guy's Hospital, London SE1 9RT, UK. thimios.mitsiadis@kcl.ac.uk
Abstract:
Notch receptors are involved in cell fate decisions through the process of lateral inhibition or inductive signalling. Jagged2 belongs to the family of transmembrane proteins that serve as the ligands for Notch receptors. We have analysed the expression of the Jagged2 gene in developing mouse teeth. Jagged2 expression is restricted in inner enamel epithelial cells that give rise to the ameloblasts. We have also examined the role of Jagged2 in tooth development using mutant mice that lack the domain of the Jagged2 protein required for interaction with the Notch receptors (DSL domain). Homozygous mutant mice die after birth, exhibit abnormal tooth morphology and fusions between the palatal and mandibular shelves. These results demonstrate that Notch signalling plays an essential role in tooth development.
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