Liposomal polyene antibiotics
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Published on: July 7, 2020
Agatha W K Ng1, Kishor M Wasan, Gabriel Lopez-Berestein
1Division of Pharmaceutics and Biopharmaceutics, The University of British Columbia, Vancouver, British Columbia, Canada.
Abstract:
Polyene antibiotics (i.e., amphotericin B and nystatin) have been incorporated into lipid-based delivery systems to decrease their toxicity and enhance their therapeutic index, the most common being liposomes. This chapter describes the protocols for preparing liposomal amphotericin B and determining the efficacy and toxicity of the formulations in animals. Furthermore, methods for determining the pharmacokinetics and drug distribution after administration of amphotericin B in lipid-based delivery systems are discussed. Procedures for comparing the toxicity of different amphotericin B formulations in cell culture studies are also elucidated.
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