Related Experiment Video
Updated: Aug 19, 2026
An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants
Published on: June 12, 2026
[Determination of triptolide in Tripterygium total terpenoids tablets by HPLC]
Cun-hai Pu1, Ming-fang He, Xiao-qian Zhanag
1Nanjing Zhongshan Pharmaceutical Factory, Nanjing 210012, China.
Objective:
To develop a HPLC method for determination of triptolide in tripterygium total terpenoids tablets.
Method:
A Lichrospher CN column was used with ethanol and water for gradient elution. The detection wavelength was set at 255 nm.
Result:
The linear relationship of the concentrations and peak areas was good in range of 0.742-59.4 microg x mL(-1) (r = 0.9998). The average recovery was 99.2%, RSD% = 1.7%.
Conclusion:
The method is simple, rapid and accurate and can be used for quality control of the tablets.
