[Established prolonged selenium deficiency of the SD rat animal model breed third generation in series]

To establish selenium deficiency of the breed third generation SD rats animal model in series.

A Se-deficient diet mainly based on Se-deficient yeast. Groups of six female and six male SD rats original generation (F0) were fed on one of the five diets: (1) selenium- and iodine-adequate control group (Se+I+ group. Selenium: 0.1-0.3 microg/g, iodine > or = 0.2 microg/g), (2) selenium-deficient iodine-adequate group 1 (Se-I+ group. Selenium: 0.02 microg/g, iodine > or = 0.2 microg/g), (3) selenium-deficient iodine-adequate group 2 (Se-I+ group2. Selenium: 0.005 microg/g, iodine > or = 0.2 microg/g), (4) selenium-adequate iodine-deficient group (Se+I- group. Selenium: 0.1-0.3 microg/g, iodine: 0.04 microg/g), (5) selenium- and iodine-deficient group (Se-I- group. Selenium: 0.01 microg/g, iodine: 0.04 microg/g). Selenium-deficient iodine-adequate group2 had been deled since generation 1 (F1). Serum Se and thyroid hormone concentrations, whole blood glutathione peroxidase (GSH-Px) activities, the weight, childbearing and livability were examined.

There were durative lower Se concentrations in serum and lower GSH-Px activities in blood, and the weight, childbearing and livability reduced in Se- group and Se-I- group from F0 to F3.

We have established prolonged selenium deficiency of the SD rat animal model breed third generation in series successfully.