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Related Experiment Videos

Quantitative kinetic analysis of the bacteriophage lambda genetic network.

Oren Kobiler1, Assaf Rokney, Nir Friedman

  • 1Department of Molecular Genetics and Biotechnology, Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.

Proceedings of the National Academy of Sciences of the United States of America
|February 25, 2005
PubMed
Summary

Bacteriophage lambda

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Area of Science:

  • Molecular Biology
  • Genetics
  • Systems Biology

Background:

  • The bacteriophage lambda lysis-lysogeny decision is a classic genetic network model.
  • This network utilizes feedback loops to respond to environmental cues and phage multiplicity.
  • Previous models proposed a bistable switch based on CI and Cro protein interactions.

Purpose of the Study:

  • To visualize the real-time dynamics of bacteriophage lambda's lytic and lysogenic pathways.
  • To investigate the in vivo regulation and function of key regulators CII and Q.
  • To elucidate the role of Cro in sensing phage infection levels.

Main Methods:

  • Real-time in vivo monitoring of gene regulator levels and function.
  • Quantitative analysis of CII and Q activity under different conditions.

Related Experiment Videos

  • Investigating the impact of CII and CIII on Cro-mediated decision-making.

    • Main Results:

    • Lysogenic conditions significantly delay and down-regulate Q activity.
    • CII function directly correlates with its protein levels, unlike Q, which exhibits a threshold.
    • Cro's regulation of CII and CIII is crucial for distinguishing single vs. multiple phage infections.

    Conclusions:

    • The study provides a high-resolution view of the lysis-lysogeny decision process.
    • Q protein exhibits a critical threshold for function, impacting pathway choice.
    • Cro's regulatory role in sensing phage multiplicity refines the understanding of this genetic switch.