Related Experiment Videos
Successful pancreas preservation by a perfluorocarbon-based one-layer method for subsequent pig islet isolation
Daniel Brandhorst1, Marcus Iken, Mathias D Brendel
1Third Medical Department, University Hospital, Rodthohl 6, 35385 Giessen, Germany. Daniel.Brandhorst@innere.med.uni-giessen.de
Transplantation
|February 25, 2005
Summary
A simplified one-layer method (OLM) using perfluorocarbon (PFC) effectively preserves pig pancreases for islet transplantation. This approach maintains islet quality and function, facilitating broader clinical application of PFC preservation solutions.
Area of Science:
- Organ transplantation
- Islet isolation and preservation
- Biomedical engineering
Background:
- The two-layer method (TLM) for pancreas oxygenation is established for clinical islet transplantation.
- Simplifying TLM could enable wider use of perfluorocarbon (PFC) as a preservation solution.
- This study investigates PFC in a one-layer method (OLM) for long-term pig pancreas preservation.
Purpose of the Study:
- To evaluate the efficacy of a simplified one-layer method (OLM) using perfluorocarbon (PFC) for long-term pig pancreas preservation.
- To compare the OLM with the established two-layer method (TLM) in terms of islet yield and quality.
- To assess the functional viability of islets preserved by OLM for transplantation.
Main Methods:
- Pig pancreases were preserved for 7 hours using either OLM (n=8) or TLM (n=10) after initial flushing with University of Wisconsin solution.
- Islet isolation was performed using collagenase NB-8 supplemented with neutral protease.
- Islet yield, purity, viability (trypan-blue exclusion, ATP content, mitochondrial function), and glucose-stimulated insulin secretion were assessed.
Main Results:
- OLM preservation resulted in significantly higher islet yield (3,110+/-520 IEQ) compared to TLM (2,080+/-290 IEQ) and was comparable to unstored pancreases (3,670+/-740 IEQ).
- No significant differences were found in islet purity, trypan-blue exclusion, ATP content, or mitochondrial viability between OLM and TLM groups.
- While TLM storage decreased the stimulation index, OLM-preserved islets maintained their responsiveness to glucose stimulation, similar to unstored controls.
Conclusions:
- Perfluorocarbon (PFC) can be successfully employed in a simplified one-layer method (OLM) for pig pancreas preservation.
- The OLM offers a practical alternative to TLM, potentially facilitating broader clinical application of PFC preservation.
- OLM preserves islet quality and function, demonstrating its potential for successful islet transplantation.