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[Carcinogenic effects of sterigmatocystin and deoxynivalenol in NIH mice]
Xiang-hua Huang1, Xiang-hong Zhang, Yue-hong Li
1Laboratory of Experimental Pathology, Hebei Medical University, Shijiazhuang, Hebei 050017, China.
Objective:
To further explore the carcinogenic activity of Sterigmatocystin (ST) and the possible synergistic carcinogenic effect of deoxynivalenol (DON) in NIH mice.
Methods:
NIH mice were randomly divided into 6 groups, 30 in each. Five groups of mice were given by gastric intubation ST 3 microg/kg, ST 30 microg/kg, ST 3 microg/kg + DON 1.5 microg/kg, ST 30 microg/kg + DON 1.5 microg/kg and DON 1.5 microg/kg respectively, 3 times a week for 24 weeks. The remaining group of mice was given normal saline accordingly, serving as control. All mice were fed with HPLC-confirmed mycotoxin-free food, analysis. The mice were killed and pathologically examined at 58th and 74th weeks.
Results:
No pathological changes were found in the control group of mice. Adenocarcinoma of lung was observed in 25.0%, 41.7%, 62.5%, 69.2% and 37.5% of mice given ST 3 microg/kg, ST 30 microg/kg, ST 3 microg/kg + DON 1.5 microg/kg, ST 30 microg/kg + DON 1.5 microg/kg and DON 1.5 microg/kg, respectively. In addition, dysplasia of glandular stomach was detected in 50.0%, 58.3%, 37.5%, 53.8% and 25.0% of mice similarly treated.
Conclusion:
Oral administration of ST or DON can induce adenocarcinoma in lung and dysplasia of glandular stomach in NIH mice. There is synergistic carcinogenic effect when both ST and DON are given.
Insights
Sterigmatocystin (ST) and deoxynivalenol (DON) cause lung adenocarcinoma and stomach dysplasia in mice. Co-administration of ST and DON exhibits a synergistic carcinogenic effect, highlighting their combined toxicity.
Area of Science:
- Toxicology
- Carcinogenesis
- Mycotoxicology
Context:
- Mycotoxins are toxic secondary metabolites produced by fungi, posing risks to human and animal health.
- Sterigmatocystin (ST) and deoxynivalenol (DON) are common mycotoxins found in food and feed.
- Understanding their individual and combined carcinogenic potential is crucial for risk assessment.
Purpose:
- To investigate the carcinogenic activity of Sterigmatocystin (ST) in NIH mice.
- To evaluate the potential synergistic carcinogenic effect of deoxynivalenol (DON) when co-administered with ST.
- To determine the specific pathological changes induced by ST and DON exposure in mice.
Summary:
- NIH mice were exposed to varying doses of ST and DON, individually and in combination, via gastric intubation for 24 weeks.
- Pathological examination revealed lung adenocarcinoma and glandular stomach dysplasia in mice treated with ST and/or DON.
- A synergistic carcinogenic effect was observed when ST and DON were administered together, indicated by increased tumor incidence.
Impact:
- This study provides evidence for the carcinogenicity of ST and DON in mice.
- The findings highlight the significant synergistic effect of combined ST and DON exposure.
- Results contribute to a better understanding of mycotoxin-induced carcinogenesis and inform risk management strategies.
