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Related Experiment Videos

Challenges in creating a vaccine to prevent hepatitis E.

Bryan J Maloney1, Naokazu Takeda, Yuriko Suzaki

  • 1Boyce Thompson Institute for Plant Research, Ithaca, NY, USA.

Vaccine
|March 1, 2005
PubMed
Summary

Transgenic potatoes expressing hepatitis E virus capsid protein (HEV CP) accumulated the protein but failed to produce immunogenic virus-like particles (VLPs). This suggests VLP assembly is crucial for oral HEV CP vaccines.

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Area of Science:

  • Biotechnology
  • Virology
  • Plant Molecular Biology

Background:

  • Recombinant hepatitis E virus capsid protein (HEV CP) can form orally immunogenic virus-like particles (VLPs) in insect cells.
  • Plant-based expression systems offer a potential platform for producing vaccine antigens.

Purpose of the Study:

  • To investigate the expression of HEV CP in transgenic potato plants.
  • To assess the assembly of HEV CP into VLPs in potato tubers.
  • To evaluate the oral immunogenicity of HEV CP expressed in potatoes.

Main Methods:

  • Transformation of potato plants with plant expression cassettes (pHEV101, pHEV110) to express HEV CP.
  • ELISA and Western blot analysis to detect and quantify HEV CP accumulation in tubers.
  • Oral immunization of mice with transgenic potatoes and assessment of anti-CP antibody response.

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Main Results:

  • Transgenic potato lines accumulated HEV CP at levels ranging from 5 to 30 microg/g fresh tuber.
  • Tuber yield was relatively consistent across lines (600-1000 g per pot).
  • Limited VLP assembly was observed in potato tubers, and oral immunization failed to elicit detectable antibody responses.

Conclusions:

  • While HEV CP can be expressed in potato tubers, VLP assembly is significantly impaired compared to insect cell systems.
  • The lack of VLP formation in potato tubers correlates with the absence of an oral immune response.
  • VLP assembly is a critical factor for the efficacy of orally delivered HEV CP vaccines, and potato expression systems may not be suitable for this purpose.