Related Experiment Video

Updated: Feb 10, 2026

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells
08:11

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Published on: August 7, 2021

4.7K

The tumor suppressor DAL-1/4.1B modulates protein arginine N-methyltransferase 5 activity in a substrate-specific

Wei Jiang1, Martha E Roemer, Irene F Newsham

  • 1David and Doreen Hermelin Laboratory of Molecular Oncogenetics, Department of Neurosurgery, Hermelin Brain Tumor Center, Henry Ford Hospital, Detroit, MI 48202, USA.

Biochemical and Biophysical Research Communications
|March 2, 2005
PubMed

Insights

The tumor suppressor DAL-1/4.1B interacts with protein arginine N-methyltransferase 5 (PRMT5), modulating its activity. This interaction impacts protein methylation, suggesting a role in controlling tumorigenesis.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • DAL-1/4.1B is a known tumor suppressor protein implicated in lung, breast, and meningioma pathogenesis.
  • Protein arginine N-methyltransferases (PRMTs) regulate crucial cellular processes including signal transduction and transcription.
  • PRMT3 was previously identified as a binding partner of DAL-1/4.1B.

Purpose of the Study:

  • To investigate the impact of DAL-1/4.1B binding on the enzymatic activity of PRMT5.
  • To explore the functional consequences of the DAL-1/4.1B-PRMT5 interaction in vitro and in cellular contexts.

Main Methods:

  • Yeast two-hybrid interaction cloning was employed to identify interacting proteins.
  • In vitro assays were conducted to assess PRMT5 activity.
  • Cellular experiments were performed to evaluate the impact of DAL-1/4.1B on PRMT5-mediated methylation.

Main Results:

  • DAL-1/4.1B was found to modulate PRMT5 activity, unlike its interaction with PRMT3.
  • PRMT5 activity was either inhibited or enhanced by DAL-1/4.1B, depending on the substrate.
  • Specific effects included inhibition of Sm protein methylation and enhancement of myelin basic protein methylation.

Conclusions:

  • The interaction between the tumor suppressor DAL-1/4.1B and PRMT5 represents a novel regulatory mechanism.
  • This interaction plays a significant role in controlling tumorigenesis through modulation of protein methylation.
  • Understanding this interplay may offer new therapeutic strategies for cancer treatment.

More Related Videos

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity09:33

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity

Published on: January 5, 2016

10.3K
Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays08:48

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

14.4K

Related Concept Videos

Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
6.1K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.9K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
9.7K
5-Number Summary01:04

5-Number Summary

In a dataset, the 5-number summary includes the minimum data value, the data value of the first quartile, the median data value or data value of the second quartile, the data value of the third quartile, and the maximum data value. These 5 data values can be visualized as a box and whisker plot.
In a box plot, the minimum and maximum data values represent the lower and upper whiskers in the graph, and the median is designated as the center of the box in the chart. The first quartile and third...
5.7K
Activation and Inactivation of G Proteins01:22

Activation and Inactivation of G Proteins

Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
11.6K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.9K