[Analysis of vitamin K1 in Cephalanoplos segetum (Bunge) Kitam by high performance liquid chromatography]

A modified method for the analysis of vitamin K1 in Cephalanoplos segetum (Bunge) Kitam, an edible wild herb, is proposed in this paper. The ground herb sample was extracted with CHCl3, followed by evaporation to dryness with nitrogen. The residue was dissolved with methanol, filtered through 0.5microm micropore membrane and then determined on a Novapak C18, 150mm x 3.9mm i.d., column with methanol/isopropanol (70:30) as mobile phase and UV-248nm as detector. Recovery was more than 95% with CV 1.23%. The method is simple and accurate without saponification, extraction and evaporation procedures as those used previously.