Related Experiment Video
Updated: Aug 19, 2026

Preparation of Cross-Linked Sodium Alginate Microspheres with Different Metal Ions Using the Microfluidic Electrospray Technology
Published on: June 7, 2024
[High performance gel chromatographic behaviour of sodium alginate and determination of its molecular weight
Abstract:
The molecular weight parameters of sodium alginate were determined by high performance gel chromatography with Waters Bondagel E-High A column. The differences in high performance gel chromatographic behaviours between the sodium alginate and the non-polar standard Dextran T500 were compared. The influences of the sample concentration and the sample volume on the results were discussed. The high performance gel chromatographic behaviour of sodium alginate is different from that of Dextran T500, and is also different from those on Sepharose or Sephadex columns. The peak shifted toward full osmotic side under in crease of ionic strength. The elution volumn didn't tend toward stable, even though at the high concentration of 0.5mol/L NaCl. A change of the sample concentration at stable sample volume or the sample volume at stable sample concentration would cause tailing peaks. It was showed that the sample size of sodium alginate would be less than 25microg, and the relative standard deviations of Mn, Mw and d were 0.95%, 4.04% and 4.70%.
Related Concept Videos
DNA Agarose Gel Electrophoresis
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Determination of Molar Masses of Polymers II
