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Published on: December 12, 2017
Immobilized IDA-type Cu2+-chelating membrane affinity chromatography for purification of bovine liver catalase
1National Chromatographic R. & A. Center of China, Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian, 116011.
Abstract:
Cu2+-chelating membrane medium based on iminodiacetate-substituted modified short cotton cellulose was examined to purify bovine liver catalase (BLC) by affinity chromatography. The buffer pH notably affect BLC binding to the Cu2+-chelating membrane adsorbent. The results showed that under the chromatographic conditions chosen, 67.7% recovery of BLC attained with an overall 4.7-fold increase in specific activity in a single step. After purification of BLC, the chelating membrane adsorbent can easily be regenerated by imidazole or EDTA buffer. Different results were obtained by the two regeneration methods: the later is more effective than the former in reviving the purification characters of the chelating membrane adsorbent.
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