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Imaging chirality with surface second harmonic generation microscopy.

Matthew A Kriech1, John C Conboy

  • 1Department of Chemistry, University of Utah, 315 S. 1400 E. RM 2020, Salt Lake City, Utah 84112, USA.

Journal of the American Chemical Society
|March 3, 2005
PubMed
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Researchers developed a novel nonlinear chiral microscope using chiral second harmonic generation (C-SHG) to image molecular chirality. This technique successfully visualized chiral patterns in lipid bilayers, overcoming limitations of traditional optical methods.

Area of Science:

  • Surface science
  • Nonlinear optics
  • Chirality imaging

Background:

  • Chirality is a fundamental property of molecules, crucial in biological and chemical systems.
  • Bulk chiral properties are measurable, but imaging molecular chirality at surfaces is challenging.
  • Linear optical methods like CD and ORD lack the necessary surface sensitivity for molecular films.

Purpose of the Study:

  • To develop and demonstrate the first nonlinear chiral microscope for imaging molecular surface chirality.
  • To visualize the intrinsic chirality of R- and S-(+)-1,1'-bi-2-naphthol (RBN, SBN) in a supported lipid bilayer.
  • To assess the surface selectivity and sensitivity of chiral second harmonic generation (C-SHG) for interfacial chirality detection.

Main Methods:

  • Development of a nonlinear chiral microscope utilizing chiral second harmonic generation (C-SHG).

Related Experiment Videos

  • Employing a counter-propagating optical geometry for enhanced surface sensitivity.
  • Imaging a patterned planar supported lipid bilayer (PSLB) of POPC with intercalated RBN or SBN molecules.
  • Comparison of C-SHG images with fluorescence microscopy for validation.

    • Main Results:

    • The C-SHG microscope successfully imaged patterned chiral structures within a POPC lipid bilayer.
    • Spatial resolution was achieved when chiral RBN or SBN molecules were present.
    • No image was formed when a racemic mixture of RBN and SBN was used, confirming chirality detection.
    • C-SHG demonstrated high surface selectivity and sensitivity for interfacial chirality.

    Conclusions:

    • Chiral second harmonic generation (C-SHG) is a viable technique for visualizing molecular chirality at interfaces.
    • The developed nonlinear chiral microscope provides a direct route for imaging chirality in molecular surface films.
    • This advancement opens new possibilities for studying chiral phenomena in complex systems.