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Related Experiment Videos

A new surface plasmon resonance sensor for high-throughput screening applications.

Marek Piliarik1, Hana Vaisocherová, Jirí Homola

  • 1Institute of Radio Engineering and Electronics, Academy of Sciences of the Czech Republic, Chaberská 57, 18251 Prague, Czech Republic.

Biosensors & Bioelectronics
|March 3, 2005
PubMed
Summary

This study introduces a novel high-throughput surface plasmon resonance (SPR) sensor. The sensor offers high-contrast imaging and precise detection for biosensing applications.

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Area of Science:

  • Biomedical Engineering
  • Optics and Photonics
  • Analytical Chemistry

Background:

  • Surface Plasmon Resonance (SPR) is a label-free optical technique widely used for detecting molecular interactions.
  • Existing SPR systems can face challenges with throughput, crosstalk, and image analysis.
  • Advancements in SPR imaging and sensor design are crucial for high-throughput biological and chemical analysis.

Purpose of the Study:

  • To develop and characterize a novel high-throughput SPR sensor.
  • To evaluate the sensor's performance in refractometry and biosensing.
  • To demonstrate the advantages of combining SPR imaging with polarization contrast and patterned multilayer structures.

Main Methods:

  • Integration of SPR imaging with polarization contrast.

Related Experiment Videos

  • Development of a spatially patterned multilayer SPR structure.
  • Utilizing a laboratory prototype with 108 sensing channels.

    • Main Results:

    • Achieved high-contrast SPR images suitable for automated analysis.
    • Demonstrated minimum crosstalk between adjacent sensing channels.
    • Obtained a refractive index resolution of 3 x 10(-6) RIU in refractometry.
    • Successfully detected human choriogonadotropin (hCG) at concentrations below 500 ng/ml.

    Conclusions:

    • The developed high-throughput SPR sensor offers significant advantages for label-free detection.
    • The system exhibits excellent performance in both refractometry and biosensing.
    • This technology has potential for sensitive and efficient molecular detection in various applications.