Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cationic polysaccharides as antiprion agents.

Ira Yudovin-Farber1, Tony Azzam, Esther Metzer

  • 1Department of Medicinal Chemistry and Natural Products, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 91120, Israel.

Journal of Medicinal Chemistry
|March 4, 2005
PubMed
Summary

Novel cationic polysaccharides effectively eliminate prion protein (PrPSc) in infected cells. Dextran-spermine showed potent activity, reducing PrPSc to undetectable levels, highlighting polycations as promising antiprion agents.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Redox- and Photo-Responsive Fe<sup>3+/2+</sup>-Cross-Linked Carboxymethyl Cellulose Methacrylate Dissipative Gels: Synthesis and Applications.

ACS applied materials & interfaces·2026
Same author

Solid Pro-Nano Lipid Oral Formulations for Cannabidiol (CBD).

Pharmaceutics·2026
Same author

Injectable Cisplatin-Loaded Biodegradable Poly(anhydride-ester) for Treating Head and Neck Cancer: Preclinical Studies.

ACS biomaterials science & engineering·2026
Same author

Toward FGF2 reduction in cultured meat media: polyphenol salts enhance growth and differentiation of bESC aggregates.

Frontiers in nutrition·2025
Same author

Effective head and neck cancer treatment combines radiation and local extended cisplatin release.

Journal of controlled release : official journal of the Controlled Release Society·2025
Same author

Synergizing Experimentation and Computation: Predicting Energetic Potential in New Cyclo-Peroxide Compounds.

ACS omega·2024

Area of Science:

  • Biochemistry
  • Neuroscience
  • Materials Science

Background:

  • Prion diseases are fatal neurodegenerative disorders caused by misfolded prion proteins (PrPSc).
  • Current treatments are limited, necessitating the development of novel therapeutic strategies.
  • Polycations have shown potential in eliminating PrPSc in vitro.

Purpose of the Study:

  • To synthesize and evaluate cationic polysaccharides for their antiprion activity.
  • To investigate the structure-activity relationship of these compounds in eliminating protease-resistant PrP (PrPSc).
  • To assess the impact of modifications on the antiprion efficacy of potent candidates.

Main Methods:

  • Synthesis of cationic polysaccharides via reductive amination of oxidized polysaccharides with oligoamines.

Related Experiment Videos

  • Testing antiprion activity by measuring the elimination of PrPSc from chronically infected neuroblastoma cells (ScN2a-M).
  • Quantification of proteinase K (PK)-resistant PrP levels and evaluation of modifications with oleic acid and methoxypoly(ethylene glycol) (MPEG).

    • Main Results:

    • Dextran-spermine demonstrated potent antiprion activity, reducing PrPSc to undetectable levels at 31 ng/mL after 4 days.
    • Oligamine identity significantly influenced PrPSc elimination, irrespective of the polysaccharide backbone.
    • Modifications with oleic acid or MPEG slightly decreased the antiprion activity of dextran-spermine.

    Conclusions:

    • Cationic polysaccharides, particularly dextran-spermine, are effective in eliminating PrPSc in vitro.
    • The chemical structure of the grafted oligoamine is crucial for antiprion efficacy.
    • Further studies on modified polycations may offer insights into developing prion disease therapeutics.