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Generation of Marked and Markerless Mutants in Model Cyanobacterial Species
Published on: May 29, 2016
Light-dependent expression of superoxide dismutase from cyanobacterium Synechocystis sp. strain PCC 6803
1Department of Biochemistry and Biotechnology Bio-Med RRC, Paichai University, 439-6 Doma 2-dong Seo-gu, Daejon 302-735, Korea.
Abstract:
The oxygenic phototrophic cyanobacterium Synechocystis sp. strain PCC 6803 inevitably evolves superoxide during photosynthesis. Synechocystis 6803 contains only one type of superoxide dismutase, designated as SodB; therefore, this protein plays an important role in preventing oxidative damages caused by light. Because there was no direct evidence that SodB in Synechocystis 6803 could be regulated by light, the relationship between SodB and light was investigated in the present study. The activity of SodB from the cells grown in continuous light culture was about 3.5-fold higher than that from the cells cultivated in continuous dark. Illumination maximally activated SodB within 12 h. The level of sodB mRNA increased 12-fold by light, and that of SodB protein proportionally. Therefore, the expression and activity of SodB from Synechocystis 6803 were dependent on the light.
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