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Related Experiment Videos

Developmental expression of Pod 1 in Xenopus laevis.

Subreena Simrick1, Karine Massé, Elizabeth A Jones

  • 1Molecular Physiology, Department of Biological Sciences, Warwick University, Coventry, UK.

The International Journal of Developmental Biology
|March 4, 2005
PubMed
Summary

Pod 1, a transcription factor, is expressed in developing mouse organs, particularly the kidney. Xenopus laevis Pod 1 shows high similarity to vertebrate versions, with expression beginning early in pronephros development.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Pod 1 is a basic helix-loop-helix transcription factor.
  • It is expressed in the mesenchyme of developing mouse organs like the heart, lungs, and gut.
  • Pod 1 is highly expressed in developing mouse kidneys, specifically in the metanephric mesenchyme, stromal cells, and podocytes.

Purpose of the Study:

  • To investigate the expression pattern of Pod 1 in Xenopus laevis during development.
  • To analyze the sequence similarity of Xenopus laevis Pod 1 to vertebrate homologues.
  • To identify the specific tissues and developmental stages where Pod 1 is expressed in Xenopus.

Main Methods:

  • Obtained a Xenopus laevis Pod 1 EST (CF270487).
  • Performed conceptual translation to assess sequence similarity.

Related Experiment Videos

  • Utilized Reverse Transcription Polymerase Chain Reaction (RT-PCR) to analyze expression levels.
  • Employed in situ hybridization to determine spatial expression patterns.

    • Main Results:

    • Xenopus laevis Pod 1 sequence shows ~85% similarity to vertebrate homologues.
    • Expression begins at stage 13 and is upregulated in the developing pronephros.
    • Higher expression in the glomus compared to tubule or duct at stage 42.
    • In situ hybridization revealed intense staining in the anterior branchial arch and pronephric glomus at tail bud stages.
    • Expression persists in the glomus and heart epicardium at stage 40.
    • Adult expression is highest in the rectum and spleen, with significant levels in other organs.

    Conclusions:

    • Xenopus laevis Pod 1 is a conserved transcription factor with a distinct expression profile during embryonic development.
    • Its expression in the pronephros, particularly the glomus, suggests a role in kidney development.
    • Pod 1 is also expressed in other developing and adult organs, indicating diverse functions.