Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Laboratory diagnosis in ulcerative keratitis.

B Khanal1, M Deb, A Panda

  • 1Department of Microbiology, B.P. Koirala Institute of Health Sciences, Dharan, Nepal.

Ophthalmic Research
|March 5, 2005
PubMed
Summary

This study identified common bacterial and fungal causes of corneal ulcers in Nepal, finding Aspergillus and Fusarium species most frequent fungi, and Staphylococcus aureus the leading bacterium. Most bacterial isolates showed sensitivity to common antibiotics, highlighting the need for ongoing surveillance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Investigating medicinal resource combinations in the Bornean orangutan diet.

Scientific reports·2026
Same author

Hidden burden of primary Epstein-Barr virus (EBV) infection in pediatric febrile illness (0-12 years) - A retrospective study.

Journal of postgraduate medicine·2026
Same author

The G.R.A.P.E. Checklist for Students of Healthcare to Finetune and Safeguard their Scholarly Manuscripts.

Kathmandu University medical journal (KUMJ)·2025
Same author

Optimizing precision conservation with on-farm precision experiment data: The role of crop insurance and spatially variable profit.

The Science of the total environment·2025
Same author

Evaluation of Latex Agglutination Test for Rapid Identification of Staphylococcus aureus Isolated from Pyogenic Wound Infections at a Tertiary Care Hospital.

Kathmandu University medical journal (KUMJ)·2023
Same author

Low yield but high levels of multidrug resistance in urinary tract infections in a tertiary hospital, Nepal.

Public health action·2021

Area of Science:

  • Ophthalmology
  • Microbiology
  • Infectious Diseases

Background:

  • Corneal ulcers are a significant cause of visual impairment globally.
  • Identifying causative agents and their antimicrobial susceptibility is crucial for effective treatment.

Purpose of the Study:

  • To determine common bacterial and fungal isolates from corneal ulcers.
  • To assess antimicrobial susceptibility patterns of bacterial isolates.
  • To compare direct microscopy with culture results for corneal ulcer diagnosis.

Main Methods:

  • Prospective evaluation of patients with suspected corneal ulceration.
  • Corneal scrapings processed for direct microscopy and microbial culture.
  • Antimicrobial susceptibility testing performed on bacterial isolates.

Related Experiment Videos

Main Results:

  • Culture positivity was achieved in 67.8% of specimens.
  • Fungal isolates included Aspergillus spp. (38.4%) and Fusarium spp. (22%).
  • Bacterial isolates were dominated by Staphylococcus aureus (56.7%) and Streptococcus pneumoniae (20%).
  • Most bacterial isolates demonstrated sensitivity to commonly used antibiotics.

Conclusions:

  • Continued surveillance of microbial agents and their susceptibility is vital for managing corneal ulcers.
  • Accurate diagnosis through microscopy and culture aids in appropriate treatment strategies.
  • Understanding local resistance patterns is essential for preventing complications.