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[Quantification of human ermap by using real-time FQ-PCR]
Xiao-Hong Zhang1, Tie-Zhen Ye, Bin Hu
1Department of Pediatrics, Conghua Central Hospital, Guangzhou 510089, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi
|March 8, 2005
Summary
This study developed a real-time quantitative PCR method for human ermap quantification. The established method is specific, sensitive, and accurate for further research on human ermap.
Area of Science:
- Molecular Biology
- Genetics
Background:
- Human ermap plays a role in biological processes.
- Accurate quantification of human ermap is crucial for research.
Purpose of the Study:
- To develop a real-time quantitative PCR (FQ-PCR) method for human ermap.
- To establish a reliable method for quantifying human ermap DNA.
Main Methods:
- Designed primers and a fluorescent probe using Primer Express 2.0.
- Generated calibration standards from ermap cDNA using pBluescriptSK(+) plasmid.
- Established and validated the real-time FQ-PCR assay.
Main Results:
- Demonstrated a strong correlation (R² = -0.999376) between template concentration and cycle threshold.
- The assay showed good linearity across a wide DNA concentration range (1.725 x 10^7 to 1.725 x 10^10 cps/ml).
Conclusions:
- The developed real-time FQ-PCR method is specific, sensitive, and accurate.
- This method is suitable for further research involving human ermap quantification.